Product Name :
3,4 Dichloroisocoumarin (CAS 51050-59-0)
Synonym :
3,4-Dichloro-2-benzopyran-1-one
Application :
3,4 Dichloroisocoumarin is a granzyme, cathepsin G, and neutrolphil elastase inhibitor
CAS:
51050-59-0
Purity:
Molecular Weight:
215.03
Formula :
C9H4Cl2O2
Physical state:
Solid
solubility :
Soluble in DMF (10 mM), DMSO (10 mM), and Ethylacetate (~50 mg/ml).
Shipping Condition :
Store at -20° C
Melting point:
SMILES:
ClC1=C(Cl)C2=CC=CC=C2C(=O)O1
References:
:The human cytotoxic T cell granule serine protease granzyme H has chymotrypsin-like (chymase) activity and is taken up into cytoplasmic vesicles reminiscent of granzyme B-containing endosomes. | Edwards, KM., et al. 1999. J Biol Chem. 274: 30468-73. PMID: 10521426A 3,4-dichloroisocoumarin-resistant component of the multicatalytic proteinase complex. | Cardozo, C., et al. 1992. Biochemistry. 31: 7373-80. PMID: 15109273,4-dichloroisocoumarin-induced activation of the degradation of beta-casein by the bovine pituitary multicatalytic proteinase complex. | Pereira, ME., et al. 1992. J Biol Chem. 267: 7949-55. PMID: 1560024Human and murine cytotoxic T lymphocyte serine proteases: subsite mapping with peptide thioester substrates and inhibition of enzyme activity and cytolysis by isocoumarins. | Odake, S., et al. 1991. Biochemistry. 30: 2217-27. PMID: 1998680Catalytic mechanism of rhomboid protease GlpG probed by 3,4-dichloroisocoumarin and diisopropyl fluorophosphonate. | Xue, Y. and Ha, Y. 2012. J Biol Chem. 287: 3099-107. PMID: 221306713,4-Dichloroisocoumarin, a serine protease inhibitor, inactivates glycogen phosphorylase b. | Rusbridge, NM. and Beynon, RJ. 1990. FEBS Lett. 268: 133-6. PMID: 2384149Mechanism-based isocoumarin inhibitors for serine proteases: use of active site structure and substrate specificity in inhibitor design. | Powers, JC., et al. 1989. J Cell Biochem. 39: 33-46. PMID: 2654146Reaction of serine proteases with substituted isocoumarins: discovery of 3,4-dichloroisocoumarin, a new general mechanism based serine protease inhibitor. | Harper, JW., et al. 1985. Biochemistry. 24: 1831-41. PMID: 3893537Inhibition of a tumour protease with 3,4-dichloroisocoumarin, pentamidine-isethionate and guanidino derivatives. | Anees, M. and Steven, FS. 1994. J Enzyme Inhib. 8: 213-21.6482-24-2 Synonym PMID: 7539490Human granzyme B is essential for DNA fragmentation of susceptible target cells.616-47-7 Formula | Su, B.PMID:33395735 , et al. 1994. Eur J Immunol. 24: 2073-80. PMID: 8088328Apparent formation of sodium dodecyl sulfate-stable complexes between serpins and 3,4-dichloroisocoumarin-inactivated proteinases is due to regeneration of active proteinase from the inactivated enzyme. | Olson, ST., et al. 1997. J Biol Chem. 272: 13338-42. PMID: 9148956Serine protease inhibitors block neutral sphingomyelinase activation, ceramide generation, and apoptosis triggered by daunorubicin. | Mansat, V., et al. 1997. FASEB J. 11: 695-702. PMID: 9240970Camptothecin-induced apoptosis in p53-null human leukemia HL60 cells and their isolated nuclei: effects of the protease inhibitors Z-VAD-fmk and dichloroisocoumarin suggest an involvement of both caspases and serine proteases. | Shimizu, T. and Pommier, Y. 1997. Leukemia. 11: 1238-44. PMID: 9264376Production of fully active recombinant murine granzyme B in yeast. | Pham, CT., et al. 1998. J Biol Chem. 273: 1629-33. PMID: 9430705