Product Name :
p21-ARC Rabbit Polyclonal Antibody
Predicted band size :
Observed band size :
Synonyms:
Actin related protein 2/3 complex subunit 3 ARC21 ARPC3_HUMAN ARP2/3 complex 21 kDa subunit ARP2/3 protein complex subunit p21 ARPC3.
Function :
p21-ARC is part of a complex implicated in the control of actin polymerization in cells. It belongs to a complex composed of ARP2, ARP3, ARPC1, ARPC2, ARPC3, ARPC4 and ARPC5, collectively known as the ARPC3 family. p21-ARC is implicated as an important regulator of growth cone translocation, especially in the CNS. Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
Antibody Type:
Rabbit Polyclonal Antibody
Immunogen :
KLH conjugated synthetic peptide derived from human p21-ARC 2-100/178
Species reactivity:
Human, Mouse, Rat, Chicken, Cow, Rabbit, Xenopus laevis
Verify application:
WB, ELISA, IHC-P, IF, ICC
Coupling :
unconjugated
Clone number:
RRID:
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at -20℃ for one year.N-Hydroxysulfosuccinimide (sodium) Purity Avoid repeated freeze/thaw cycles.Fmoc-Dab(Alloc)-OH site The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃.PMID:33586434 When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage buffer solution:
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
20 KDa
Positive control :
Mouse intestinal lysate, Raji cell lysate, Mouse Spleen Lysate.